Trace elements solution
Internal Solution-ID: S1935
This solution is used in 2 media and was first defined in medium 939.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 50.00 | g | 50 | 171.092 | |
| ZnSO4 x 7 H2O | 1.00 | g | 1 | 3.478 | |
| CaCl2 x 2 H2O | 7.34 | g | 7.34 | 49.927 | |
| MnCl2 x 4 H2O | 2.50 | g | 2.5 | 12.632 | |
| CoCl2 x 6 H2O | 0.50 | g | 0.5 | 3.851 | |
| (NH4)6Mo7O24 | 0.50 | g | 0.5 | - | |
| FeSO4 x 7 H2O | 5.00 | g | 5 | 17.985 | |
| CuSO4 x 5 H2O | 0.20 | g | 0.2 | 0.801 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve the EDTA in about 400 ml of water, then add 9 g NaOH. Dissolve each of the salts individually in about 40-50 ml of water and add them to the EDTA-NaOH solution. Adjust the final pH of the solution to pH 6.0 with 1M NaOH (about 24 ml). Make up to 1 litre with distilled water, store in the dark and do not autoclave the stock solution before it is added to the medium. | |||||