Main sol. 946
Internal Solution-ID: S1957
Equipment needed: Hungate tubes
Filter
Gassing station
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Synthetic seawater (2 x conc.) | 500.00 | ml | - | - | |
| NH4Cl | 0.33 | g | 0.326 | 6.102 | |
| KH2PO4 | 0.33 | g | 0.326 | 2.399 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
Sulfur
(powdered) |
10.00 | g | 9.891 | 308.425 | |
| Na2CO3 | 1.50 | g | 1.484 | 13.999 | |
| Na-formate | 3.00 | g | 2.967 | 43.633 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.60 | g | 0.593 | 2.471 | |
| Distilled water | 500.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, carbonate, formate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur. For sterilization heat cultivation vessels for 1 - 2 hours to 90 - 100°C in a water bath on each of three successive days. Prior to inoculation add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and formate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.8, if necessary. | |||||
Media with this solution
| 946 | NAUTILIA MEDIUM |