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Solutions A, B and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121⁰C for 15 min. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. For solid medium add 15.0 g/l agar to solution A.
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