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Main sol. 962

Internal Solution-ID: S1985

Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-9,10-anthraquinone-2,6-disulfonate
(TCI A0308)
8.00 g 7.913 -
NH4Cl 0.33 g 0.326 6.102
KH2PO4 0.33 g 0.326 2.399
MgCl2 x 6 H2O 0.33 g 0.326 1.606
CaCl2 x 2 H2O 0.33 g 0.326 2.22
KCl 0.33 g 0.326 4.378
Yeast extract
(OXOID)
0.05 g 0.049 -
Modified Wolin's mineral solution 10.00 ml - -
NaHCO3 2.50 g 2.473 29.436
Na-acetate 3.00 g 2.967 36.172
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, acetate and vitamins), boil medium to dissolve AQDS, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Add solid bicarbonate and adjust pH to 6.8 - 7.0. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
962 THERMINCOLA FERRIACETICA MEDIUM