Main sol. 964
Internal Solution-ID: S1988
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
30.00 | g | 30 | - | |
|
PIPES buffer
(SIGMA) |
6.05 | g | 6.05 | 20.009 | |
| D-Glucose | 5.00 | g | 5 | 27.753 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
|
Sulfur
(powdered) |
12.00 | g | 12 | 374.181 | |
|
Peptone
(BD Bacto) |
1.00 | g | 1 | - | |
|
Yeast extract
(BD Bacto) |
0.50 | g | 0.5 | - | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, peptone, yeast extract, and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.2 - 7.4, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 110°C for 20 min. Add peptone, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 7.5. | |||||
Media with this solution
| 964 | CAMINICELLA MEDIUM |