Main sol. 965
Internal Solution-ID: S1989
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.99 | 18.51 | |
| K2HPO4 | 0.30 | g | 0.297 | 1.705 | |
| KH2PO4 | 0.30 | g | 0.297 | 2.183 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.495 | 2.435 | |
| NaCl | 2.00 | g | 1.98 | 33.884 | |
| KCl | 0.20 | g | 0.198 | 2.656 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.337 | |
|
Yeast extract
(OXOID) |
1.00 | g | 0.99 | - | |
|
Tryptone
(BD Bacto) |
2.00 | g | 1.98 | - | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Starch
(soluble) |
10.00 | g | 9.901 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | 2.819 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 4.951 | 19.947 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and thiosulfate), bring medium to the boil then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.5, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add thiosulfate from a sterile anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.5, if necessary. | |||||
Media with this solution
| 965 | THERMOANAEROBACTER KREATINOPHILUS MEDIUM |