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Main sol. 976

Internal Solution-ID: S2001

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
KNO3 1.00 g 1 9.891
MgSO4 x 7 H2O 0.22 g 0.22 0.893
NaCl 30.00 g 30 513.347
Na2CO3 5.00 g 5 47.175
Trace elements solution 1.00 ml - -
Distilled water 1000.00 ml - -
Methanol 10.00 ml 7.92 247.176
1 Final pH 9.5
2 Prepare the medium without the Na2CO3, which can be sterilised separately by autoclaving. 10 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 stock solution and agar to 50-55°C before mixing.
976 METHYLOPHAGA ALCALICA MEDIUM