Main sol. 976
Internal Solution-ID: S2001
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| KNO3 | 1.00 | g | 1 | 9.891 | |
| MgSO4 x 7 H2O | 0.22 | g | 0.22 | 0.893 | |
| NaCl | 30.00 | g | 30 | 513.347 | |
| Na2CO3 | 5.00 | g | 5 | 47.175 | |
| Trace elements solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Methanol | 10.00 | ml | 7.92 | 247.176 | |
| 1 Final pH 9.5 | |||||
| 2 Prepare the medium without the Na2CO3, which can be sterilised separately by autoclaving. 10 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 stock solution and agar to 50-55°C before mixing. | |||||
Media with this solution
| 976 | METHYLOPHAGA ALCALICA MEDIUM |