1
When grown aerobically prepare the medium without the vitamins, adjust the pH to 7.0 with 10M KOH, and sterilise for 45 min at 110°C. On cooling add the vitamins from a filter sterilised stock solution. Both Geococcus nitratireducens (DSM 15495) and Petrobacter succinimandens (DSM 15512) are supplied from aerobically grown cultures.
2
Anaerobic growth may be obtained by gassing the medium with N2:CO2 (80:20), to which 10mM KNO3 has been added, and dispensing the medium under N2:CO2 (80:20). Sterilise by autoclaving at 110°C for 45 min , and to the cold medium add the filter sterilised vitamins. A reducing agent is not required.
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