Main sol. 1006
Internal Solution-ID: S2052
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| DL-Histidine | 7.80 | g | 7.769 | 50.072 | |
| Yeast extract | 4.00 | g | 3.984 | - | |
|
Trypticase peptone
(BD BBL) |
1.00 | g | 0.996 | - | |
| K2HPO4 | 0.29 | g | 0.289 | 1.658 | |
| KH2PO4 | 0.29 | g | 0.289 | 2.123 | |
| (NH4)2SO4 | 0.48 | g | 0.478 | 3.618 | |
| NaCl | 0.48 | g | 0.478 | 8.181 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.404 | |
| CaCl2 x 2 H2O | 64.00 | mg | 0.064 | 0.434 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 4.00 | g | 3.984 | 37.59 | |
| Volatile fatty acid mixture | 3.10 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.498 | 2.835 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary. | |||||
Media with this solution
| 1006 | ALLISONELLA MEDIUM |