MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1011d

Download this solution as

CSV JSON PDF

Internal Solution-ID: S2062

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.674 507.762
K2HPO4 0.14 g 0.138 0.795
CaCl2 x 2 H2O 0.14 g 0.138 0.942
MgSO4 x 7 H2O 3.40 g 3.363 13.644
MgCl2 x 6 H2O 4.18 g 4.135 20.337
KCl 0.33 g 0.326 4.378
NH4Cl 0.25 g 0.247 4.623
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Na2CO3 2.50 g 2.473 23.331
Na2S2O3 x 5 H2O 1.50 g 1.484 5.978
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, thiosulfate and vitamins), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials up to a volume of 20% and autoclave. Add carbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under 100% N2 gas, whereas the stock solution of carbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust pH of the complete medium to 6.7.
2 After inoculation pressurize vessels to 0.5 bar overpressure with sterile 100% CO2 gas and add sterile air in an amount that is equivalent to a volume of 20% of the headspace.
1011d GALENEA MJ MEDIUM (CO2)