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Main sol. 1013

Internal Solution-ID: S2064

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial sea water 700 ml - -
Modified Wolin's mineral solution 10 ml - -
Distilled water 300 ml - -
1 Mix ingredients and adjust pH of the final solution to 7.5 - 8.0. Sterilize by autoclaving at 121°C for 20 min. Grow prey bacterium (DSM 15416) on agar plates using medium 514. For 50 ml prey medium suspension use one agar plate incubated for 24 hours and wash off grown cells in 3 - 5 ml medium. The prey medium suspension should contain approx. 107 to 109 cells. Inoculate the complete medium immediately after preparation and incubate the suspension with shaking until a decrease in turbidity is visible.