Main sol. 1014
Internal Solution-ID: S2065
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 175.00 | g | 174.825 | 2991.53 | |
| K2HPO4 | 1.50 | g | 1.499 | 8.603 | |
| NH4Cl | 0.50 | g | 0.5 | 9.338 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.983 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.013 | |
| Na2S2O3 | 5.00 | g | 4.995 | 11.657 | |
| NaHCO3 | 4.00 | g | 3.996 | 47.568 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve sodium chloride, hydrogenphosphate, ammonium chloride and trace elements and fill solution in screw capped Erlenmeyer flasks to 10% of volume. Autoclave at 110°C for 30 min. Add magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5. | |||||
Media with this solution
| 1014 | THIOALKALIVIBRIO HALOPHILUS MEDIUM |