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Trace elements solution

Internal Solution-ID: S2071

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.80 g 12.8 66.967
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
FeSO4 x 7 H2O 0.10 g 0.1 0.36
MnCl2 x 4 H2O 0.10 g 0.1 0.505
NaCl 0.10 g 0.1 1.711
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
Distilled water 1000.00 ml - -
1 First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH.
1019 ROUF'S MEDIUM