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Sludge fluid

Internal Solution-ID: S208

This solution is used in 9 media and was first defined in medium 119.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.4 g 4 -
Sludge 100.0 ml - -
1 Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark.
119c GUDONGELLA MEDIUM
119b METHANOMASSILIICOCCUS MEDIUM
119 METHANOBACTERIUM MEDIUM
140 RUMINICLOSTRIDIUM MEDIUM
320 CLOSTRIDIUM CELLULOVORANS MEDIUM
321 METHANOCORPUSCULUM AGGREGANS MEDIUM
374 METHANOSPIRILLUM SK MEDIUM
1084 METHERMICOCCUS (METHANOGEN) MEDIUM
J872 METHANOBACTERIUM MEDIUM (IV)