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Main sol. 1024

Internal Solution-ID: S2082

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 1 g 1 11.765
NaHCO3 1 g 1 11.904
Na2S2O3 x 5 H2O 1 g 1 4.029
Sulfur powder 3 g 3 11.696
Wolin's vitamin solution 10 ml - -
Modified Wolin's mineral solution II 10 ml - -
Synthetic seawater 1000 ml - -
1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution.
2 Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0.
1024 NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM