Main sol. 1036
Internal Solution-ID: S2104
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.499 | 9.329 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.491 | |
| KCl | 0.2 | g | 0.2 | 2.677 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2CO3 | 1.0 | g | 0.998 | 9.416 | |
| NaHCO3 | 7.6 | g | 7.585 | 90.288 | |
| NaCl | 10.0 | g | 9.98 | 170.774 | |
| Yeast extract | 0.2 | g | 0.2 | - | |
| Cellobiose | 3.0 | g | 2.994 | 8.747 | |
| Na2S x 9 H2O | 0.5 | g | 0.499 | 2.078 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except cellobiose and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas sterilized by filtration and sulfide from a sterile anoxic stock solution autoclaved under 100% N2 gas. Adjust pH of the complete medium to pH 8.8-9.0. | |||||
Media with this solution
| 1036 | ACETIVIBRIO ALKALICELLULOSI MEDIUM |