MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1042

Download this solution as

CSV JSON PDF

Internal Solution-ID: S2110

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA) 		30.0 g 29.851 -
NaNO3 1.0 g 0.995 11.707
Wolfe's mineral elixir 2.0 ml - -
VOSO4 x 5 H2O
(0.01% w/v) 		0.5 ml 5.0e-5 2.0e-4
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
FeSO4 x 7 H2O 0.1 g 0.1 0.358
NaHCO3 1.0 g 0.995 11.845
Yeast extract 0.5 g 0.498 -
Na-pyruvate 1.0 g 0.995 9.042
Wolin's vitamin solution (10x) 2.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except ferrous sulfate, bicarbonate, yeast extract, pyruvate and vitamins), adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense the medium under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. Add ferrous sulfate, bicarbonate, yeast extract, pyruvate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Prepare the stock solution of ferrous sulfate by dissolving 1% (w/v) FeSO4 x 7 H2O in 0.1 N H2SO4. The stock solutions of pyruvate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 6.8-7.0.
2 Note: It may be necessary to add 10-20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under 100% N2 and filter-sterilized), if the medium is not completely reduced after inoculation.
1042 VULCANIBACILLUS MEDIUM