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Main sol. 1068

Internal Solution-ID: S2157

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.499 3.667
NH4Cl 1.00 g 0.998 18.658
Na2SO4 1.00 g 0.998 7.026
NaCl 10.00 g 9.98 170.774
CaCl2 x 2 H2O 0.10 g 0.1 0.679
MgSO4 x 7 H2O 2.00 g 1.996 8.098
Trace element solution SL-10 1.00 ml - -
Yeast extract 0.50 g 0.499 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Na-DL-lactate 2.50 g 2.495 22.265
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.25 1.421
Na2S x 9 H2O 0.25 g 0.25 1.039
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except lactate, vitamins and reducing agents), adjust pH to 7.5 and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, vitamins, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.5, if necessary.
1068 DESULFOPILA MSL86 MEDIUM