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Main sol. 1069

Internal Solution-ID: S2158

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.5 3.674
CaCl2 x 2 H2O 0.15 g 0.15 1.02
MgSO4 x 7 H2O 2.00 g 2 8.114
NH4Cl 0.34 g 0.34 6.356
NaCl 20.00 g 20 342.231
Yeast extract 0.40 g 0.4 -
Ferric citrate
(0.1% w/v)
5.00 ml 0.005 0.02
Trace element solution SL-7 1.00 ml - -
Distilled water 1000.00 ml - -
1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.
1069 MODIFIED BIEBL AND PFENNIG'S MEDIUM