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Main sol. 120c

Internal Solution-ID: S217

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
K₂HPO₄	0.35	g	0.349	2.001
KH₂PO₄	0.23	g	0.229	1.683
NH₄Cl	0.50	g	0.498	9.31
MgSO₄ x 7 H₂O	0.50	g	0.498	2.021
CaCl₂ x 2 H₂O	0.25	g	0.249	1.694
NaCl	2.25	g	2.241	38.348
FeSO4 x 7 H2O solution (0.1% w/v)	2.00	ml	-	-
Trace element solution SL-10	1.00	ml	-	-
Yeast extract (OXOID)	2.00	g	1.992	-
Casitone (BD BBL)	2.00	g	1.992	-
Na-acetate	10.00	g	9.96	121.415
Sucrose	17.10	g	17.032	49.757
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	0.002
NaHCO₃	2.00	g	1.992	23.713
Wolin's vitamin solution (10x)	1.00	ml	-	-
L-Cysteine HCl x H₂O	0.30	g	0.299	1.701
Na₂S x 9 H₂O	0.30	g	0.299	1.244
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 - 45 min to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N₂ and 20% CO₂ gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Cysteine and sulfide are autoclaved separately under 100% N₂ gas atmosphere as concentrated stock solutions. Vitamins are prepared under 100% N₂ gas atmosphere and sterilized by filtration. Appropriate volumes of the solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.

Compound

Amount

Unit

Conc. [g/L]

Conc. [mM]

K₂HPO₄

0.35

0.349

2.001

KH₂PO₄

0.23

0.229

1.683

NH₄Cl

0.50

0.498

9.31

MgSO₄ x 7 H₂O

0.50

0.498

2.021

CaCl₂ x 2 H₂O

0.25

0.249

1.694

NaCl

2.25

2.241

38.348

FeSO4 x 7 H2O solution (0.1% w/v)

2.00

Trace element solution SL-10

1.00

Yeast extract
(OXOID)

2.00

1.992

Casitone
(BD BBL)

2.00

1.992

Na-acetate

10.00

9.96

121.415

Sucrose

17.10

17.032

49.757

Sodium resazurin
(0.1% w/v)

0.50

5.0e-4

0.002

NaHCO₃

2.00

1.992

23.713

Wolin's vitamin solution (10x)

1.00

L-Cysteine HCl x H₂O

0.30

0.299

1.701

Na₂S x 9 H₂O

0.30

0.299

1.244

Distilled water

1000.00

1 Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 - 45 min to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N₂ and 20% CO₂ gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Cysteine and sulfide are autoclaved separately under 100% N₂ gas atmosphere as concentrated stock solutions. Vitamins are prepared under 100% N₂ gas atmosphere and sterilized by filtration. Appropriate volumes of the solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.

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Main sol. 120c

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Main sol. 120c

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