Back to all solutions

Main sol. 120d

Internal Solution-ID: S218

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
K₂HPO₄	0.35	g	0.342	1.962
KH₂PO₄	0.23	g	0.225	1.651
NH₄Cl	0.50	g	0.488	9.128
MgCl₂ x 6 H₂O	10.00	g	9.766	48.035
CaCl₂ x 2 H₂O	0.15	g	0.146	0.996
NaCl	23.00	g	22.461	384.342
FeSO4 x 7 H2O solution (0.1% w/v)	2.00	ml	-	-
Trace element solution SL-10	1.00	ml	-	-
Sodium resazurin (0.1% w/v)	0.50	ml	4.9e-4	0.002
Na₂CO₃	1.00	g	0.977	9.214
Methanol (50% v/v)	20.00	ml	15.469	482.765
Wolin's vitamin solution (10x)	1.00	ml	-	-
L-Cysteine HCl x H₂O	0.30	g	0.293	1.668
Na₂S x 9 H₂O	0.30	g	0.293	1.22
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients (except carbonate, vitamins, methanol, cysteine and sulfide) and sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 - 45 min to make it anoxic. Dispense medium under 80% N₂ and 20% CO₂ gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Methanol (50% v/v stock solution) and the reducing agents are each autoclaved separately under 100% N₂ gas atmosphere as concentrated solutions in tightly closed tubes. Carbonate is prepared under 80% N₂ and 20% CO₂ gas mixture and autoclaved separately. Vitamins are prepared under 100% N₂ gas atmosphere and sterilized by filtration. Appropriate volumes of the stock solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.

Compound

Amount

Unit

Conc. [g/L]

Conc. [mM]

K₂HPO₄

0.35

0.342

1.962

KH₂PO₄

0.23

0.225

1.651

NH₄Cl

0.50

0.488

9.128

MgCl₂ x 6 H₂O

10.00

9.766

48.035

CaCl₂ x 2 H₂O

0.15

0.146

0.996

NaCl

23.00

22.461

384.342

FeSO4 x 7 H2O solution (0.1% w/v)

2.00

Trace element solution SL-10

1.00

Sodium resazurin
(0.1% w/v)

0.50

4.9e-4

0.002

Na₂CO₃

1.00

0.977

9.214

Methanol
(50% v/v)

20.00

15.469

482.765

Wolin's vitamin solution (10x)

1.00

L-Cysteine HCl x H₂O

0.30

0.293

1.668

Na₂S x 9 H₂O

0.30

0.293

1.22

Distilled water

1000.00

1 Dissolve ingredients (except carbonate, vitamins, methanol, cysteine and sulfide) and sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 - 45 min to make it anoxic. Dispense medium under 80% N₂ and 20% CO₂ gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Methanol (50% v/v stock solution) and the reducing agents are each autoclaved separately under 100% N₂ gas atmosphere as concentrated solutions in tightly closed tubes. Carbonate is prepared under 80% N₂ and 20% CO₂ gas mixture and autoclaved separately. Vitamins are prepared under 100% N₂ gas atmosphere and sterilized by filtration. Appropriate volumes of the stock solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.

Content

Search

Tools

Main sol. 120d

Have some cookies!

Content

Search

Tools

Main sol. 120d

Media with this solution

Have some cookies!