Main sol. 1085
Internal Solution-ID: S2194
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casitone
(BD Bacto) |
20.00 | g | 20 | - | |
|
Yeast extract
(BD Bacto) |
5.00 | g | 5 | - | |
| Na2HPO4 | 5.00 | g | 5 | 35.221 | |
| MgCl2 x 6 H2O | 1.10 | g | 1.1 | 5.411 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Urea | 1.00 | g | 1 | 16.651 | |
| Na-thioglycolate | 0.75 | g | 0.75 | 6.573 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except urea and thioglycolate), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add thioglycolate, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add urea from a 10% (w/v) stock solution sterilized by filtration and stored under 100% N2 gas. Adjust pH of complete medium to 7.4. | |||||
Media with this solution
| 1085 | HOWARDELLA MEDIUM |