Main sol. 1086
Internal Solution-ID: S2195
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2HPO4 x 2 H2O | 0.53 | g | 0.528 | 3.722 | |
KH2PO4 | 0.41 | g | 0.409 | 3.004 | |
NH4Cl | 0.30 | g | 0.299 | 5.592 | |
CaCl2 x 2 H2O | 0.11 | g | 0.11 | 0.746 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.49 | |
NaCl | 0.30 | g | 0.299 | 5.118 | |
Na2SO4 | 2.80 | g | 2.792 | 19.654 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Yeast extract | 0.02 | g | 0.02 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Na2CO3 | 1.50 | g | 1.496 | 14.11 | |
Na-benzoate | 0.43 | g | 0.429 | 2.975 | |
Na-crotonate solution (1 M) | 20.00 | ml | - | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.499 | 2.076 | |
Distilled water | 980.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, benzoate, crotonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add benzoate, crotonate, vitamins and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The crotonate and vitamin solutions should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2. | |||||
2 After inoculation pressurize the vessels with sterile 80% N2 and 20% CO2 gas mixture to 0.7 bar overpressure. |
1086 | DESULFATIRHABDIUM MEDIUM |