Main sol. 1091
Internal Solution-ID: S2198
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 195.00 | g | 195 | 3336.76 | |
| MgSO4 x 7 H2O | 50.00 | g | 50 | 202.86 | |
| MgCl2 x 6 H2O | 35.00 | g | 35 | 172.157 | |
| KCl | 5.00 | g | 5 | 67.069 | |
| NaHCO3 | 0.25 | g | 0.25 | 2.976 | |
| NaNO3 | 1.00 | g | 1 | 11.765 | |
| CaCl2 x 2 H2O | 0.50 | g | 0.5 | 3.401 | |
| KH2PO4 | 0.05 | g | 0.05 | 0.367 | |
| NH4Cl | 0.03 | g | 0.03 | 0.561 | |
|
Tris buffer
(1M) |
20.00 | ml | 2.423 | 20 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add after autoclaving: | |||||
| Yeast extract | 0.05 | g | 0.05 | - | |
| Sodium pyruvate | 1.00 | g | 1 | 9.088 | |
| 2 Final pH 7.4 | |||||
| 3 Yeast extract and sodium pyruvate are added from sterile (autoclaved) stock solutions to the respective concentrations. | |||||
Media with this solution
| 1091 | HALOQUADRATUM WALSBYI MEDIUM |