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Main sol. 25

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Internal Solution-ID: S22

Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.25 g 0.25 -
Mannitol 1.50 g 1.5 8.234
Na-gluconate 0.50 g 0.5 2.292
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NH4Cl 0.40 g 0.4 7.478
NaCl 0.40 g 0.4 6.845
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL-6 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 4.9, then add:
Fe(III) citrate
(0.1% in H2O) 		5.00 ml 0.005 0.02
Biotin
(0.002% in H2O) 		1.00 ml 2.0e-5 8.2e-5
p-Aminobenzoic acid
(0.01% in H2O) 		1.00 ml 1.0e-4 7.3e-4
Sodium thiosulfate pentahydrate
(10% in H2O) 		2.00 ml 0.2 0.806
2 Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp
25 RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM