Main sol. 1108
Internal Solution-ID: S2228
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
N-Z-Case
(Wako pure chemicals, Japan = Peptone from Casein) |
3.000 | g | 3 | - | |
| Yeast extract | 2.000 | g | 2 | - | |
|
Starch
(soluble) |
1.000 | g | 1 | - | |
| NaCl | 3.000 | g | 3 | 51.335 | |
| MgCl2 | 0.125 | g | 0.125 | 1.313 | |
| CaCl2 | 0.025 | g | 0.025 | 0.225 | |
| FeSO4 x 7 H2O | 0.010 | g | 0.01 | 0.036 | |
| Gelrite | 8.000 | g | 8 | - | |
| H2O | 900.000 | ml | - | - | |
| 1 Add 100 µl each of Trace Metal solutions according to the following order: | |||||
| Na2MoO4 x 2 H2O | 12.000 | g | 12 | 49.597 | |
| VOSO4 x H2O | 1.000 | g | 1 | - | |
| MnCl2 x 4 H2O | 5.000 | g | 5 | 25.265 | |
| ZnSO4 x 7 H2O | 0.600 | g | 0.6 | 2.087 | |
| CuSO4 x 5 H2O | 0.150 | g | 0.15 | 0.601 | |
| CoCl2 x 6 H2O | 8.000 | g | 8 | 61.615 | |
| NiCl2 x 6 H2O | 0.200 | g | 0.2 | 1.543 | |
| 2 Adjust to pH 7.5 at room temperature and add water to the final volume of 1 liter. Sterilize with and autoclave at 121°C for 15 min. | |||||
Media with this solution
| 1108 | CYS MEDIUM |