Main sol. 1113
Internal Solution-ID: S2234
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Leptospira Medium Base EMJH
(Difco) |
2.3 | g | 2.3 | - | |
Agarose | 1.5 | g | 1.5 | 2.379 | |
Water | 900.0 | ml | - | - | |
Leptospira Enrichment EMJH | 100.0 | ml | - | - | |
1 Dissolve the agarose in water by boiling. Add the Leptospira base and dissolve completely. Autoclave at 121°C for 15 min. Allow the medium to cool to 50-55°C. Warm the Leptospira Enrichment to approximately 25°C and add to the warm medium. Mix uniformly and dispense aseptically into sterile tubes as requested. Final pH is 7.5 at 25°C. |
1113 | LEPTOSPIRA-MEDIUM |