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Main sol. 1113

Internal Solution-ID: S2234

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Leptospira Medium Base EMJH
(Difco)
2.3 g 2.3 -
Agarose 1.5 g 1.5 2.379
Water 900.0 ml - -
Leptospira Enrichment EMJH 100.0 ml - -
1 Dissolve the agarose in water by boiling. Add the Leptospira base and dissolve completely. Autoclave at 121°C for 15 min. Allow the medium to cool to 50-55°C. Warm the Leptospira Enrichment to approximately 25°C and add to the warm medium. Mix uniformly and dispense aseptically into sterile tubes as requested. Final pH is 7.5 at 25°C.
1113 LEPTOSPIRA-MEDIUM