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Main sol. 1114

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Internal Solution-ID: S2235

Equipment needed: Gassing station Serum bottle Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 972 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
Solution E 1 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into serum vials (to 20% of volume) and autoclave. Solution B is autoclaved separately under 100% N2 gas and solution C under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. The pH of the complete medium should be 6.5.
2 After inoculation cultivation vials are pressurized with an amount of sterile air that is equivalent to 30 vol% of the cultivation vial.
1114 THIOFABA MEDIUM