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Main sol. 1115

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Internal Solution-ID: S2241

Equipment needed: Filter Gassing station Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA) 		35.00 g 34.965 -
Wolfe's mineral elixir 1.00 ml - -
NH4Cl 0.10 g 0.1 1.868
Yeast extract 1.00 g 0.999 -
Na-pyruvate 1.10 g 1.099 9.986
HEPES
(SIGMA) 		2.50 g 2.498 10.481
L-Histidine 0.04 g 0.04 0.258
L-Cysteine HCl x H2O 0.04 g 0.04 0.228
Seven vitamins solution 0.50 ml - -
KH2PO4 0.05 g 0.05 0.367
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except vitamins and hydrogenphosphate) and adjust pH to 7.7. After autoclaving add vitamins and hydrogenphosphate from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 7.3 - 7.5, if necessary.
2 Note: Growth of most strains is stimulated by using microaerophilic conditions with only 10% (v/v) oxygen in the head space gas atmosphere. For microaerophilic growth medium is prepared without HEPES, sparged with 80% N2 and 20% CO2 gas mixture to make it anoxic and then dispensed under air atmosphere into serum vials to 50% volume. After autoclaving the pH is adjusted to 7.5 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture.
1115 CONGREGIBACTER (SYPHC) MEDIUM