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Main sol. 1118

Internal Solution-ID: S2247

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casitone 3.0 g 3 -
CaCl2 x 2 H2O 0.7 g 0.7 4.761
MgSO4 x 7 H2O 2.0 g 2 8.114
Trace element solution SL-4 1.0 ml - -
Distilled water 1000.0 ml - -
Vitamin B12 0.5 mg 5.0e-4 3.7e-4
1 After autoclaving and cooling, add 0.5 mg /l vitamin B12 from a filter sterilized stock solution. pH should be 7.0 -7.2.
1118 MD1-MEDIUM