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Main sol. 1119

Internal Solution-ID: S2249

Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium gluconate 3.00 g 3 13.753
Yeast extract
(Difco)
1.00 g 1 -
(NH4)2SO4 1.00 g 1 7.568
KH2PO4 1.00 g 1 7.348
NaCl 0.20 g 0.2 3.422
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL8 1.00 ml - -
Distilled water 1000.00 ml - -
Agar , for solid medium 1.80 % 18 53.519
1 Adjust pH to 4.0 - 4.5.
2 For solid medium, add 1.8% agar (final) and adjust pH to 5.0 to avoid hydrolysis of agar.
1119 GNYS MEDIUM