Main sol. 1130
Internal Solution-ID: S2266
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 175.0 | g | 175 | 2994.52 | |
MgCl2 x 6 H2O | 20.0 | g | 20 | 98.375 | |
K2SO4 | 5.0 | g | 5 | 28.693 | |
CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
Yeast extract
(ThermoFischer) |
5.0 | g | 5 | - | |
Distilled water | 1000.0 | ml | - | - | |
Agar
optional |
15.0 | g | 15 | - | |
1 Adjust to pH 6.8-7.2 | |||||
2 Note: for Agar plates, autoclave NaCl separately. Otherwise the salts will precipitate and the plates not solidify properly. | |||||
3 Note: especially for strains DSM 17983 and 17984, yeast extract from ThermoFisher has to be used. Yeast extract from other vendors might not support growth. |
1130 | RE-101/102 MEDIUM |