MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1132

Download this solution as

CSV JSON PDF

Internal Solution-ID: S2271

Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 0.20 g 0.2 1.36
KNO3 0.05 g 0.05 0.495
Trace element solution SL-10 1.00 ml - -
H2O 1000.00 ml - -
1 Dispense 5 ml aliquots into Hungate tubes. After autoclaving add the following components per liter:
Phosphate buffer
(pH 6.8) 		2.00 ml - -
HEPES buffer
(pH 7.0, 2 M) 		1.00 ml 0.477 2
Fe(III)NH4-EDTA
(4mg/ml) 		1.00 ml - -
Methanol 0.30 ml 0.238 7.415
2 Adjust to pH 6.8.
3 Incubate in sealed containers. The strain held in the DSMZ has been cultivated on methanol, but the original publication indicates that the strain will also grow on methane.
1132 LC 2