Main sol. 1134
Internal Solution-ID: S2275
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1 | g | 1 | 5.741 | |
| KNO3 | 1 | g | 1 | 9.891 | |
| Na2CO3 | 11 | g | 11 | 103.785 | |
| Methanol | 2 | ml | 1.584 | 49.435 | |
| NaCl | 18 | g | 18 | 308.008 | |
| MgSO4 x 7 H2O | 2 | ml | - | - | |
| NaS2O3 x 5 H2O | 18 | g | 18 | - | |
| Trace element solution SL-6 | 1 | ml | - | - | |
| Vitamin B12 | 100 | µg | 1.0e-4 | 7.4e-5 | |
| Distilled water | 1000 | ml | - | - | |
| 1 pH 10.0 | |||||
| 2 Prepare the medium without methanol, vitamin B12, MgSO4 x 7 H2O and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol. | |||||
Media with this solution
| 1134 | METHYLONATRUM MEDIUM |