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Main sol. 1136

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Internal Solution-ID: S2279

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.5 3.674
CaCl2 x 2 H2O 0.12 g 0.12 0.816
MgSO4 x 7 H2O 2.00 g 2 8.114
NH4Cl 0.64 g 0.64 11.965
NaCl 20.00 g 20 342.231
Yeast extract 0.50 g 0.5 -
Sodium pyruvate 3.00 g 3 27.263
Trace element solution SL-12 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust to pH 7.0
2 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.
1136 RHODOVULUM KHOLENSE MEDIUM