Main sol. 133
Internal Solution-ID: S229
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2HPO4 x 12 H2O | 4.50 | g | 4.5 | 31.699 | |
KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
NH4Cl | 1.50 | g | 1.5 | 28.043 | |
MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
Ferric ammonium citrate | 18.00 | mg | 0.018 | 0.069 | |
Trace element solution SL-6 | 1.00 | ml | - | - | |
Na-acetate
optional |
3.00 | g | - | - | |
Agar
, for solid medium
optional |
12.00 | g | 12 | 35.679 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients, adjust pH to 7.0 and autoclave. | |||||
2 For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% carbon monoxide + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.50 g NaHCO3 per liter of medium. | |||||
3 For chemoorganotrophic growth add 3.00 g sodium acetate and incubate under air atmosphere. |
133 | CARBON MONOXIDE OXIDIZER MEDIUM |