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Main sol. 1154

Internal Solution-ID: S2317

Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 0.150 g 0.15 0.833
(NH4)2SO4 0.500 g 0.5 3.784
CaCO3 0.100 g 0.1 0.999
Ca(NO3)2 0.100 g 0.1 0.609
KCl 0.050 g 0.05 0.671
K2HPO4 0.050 g 0.05 0.287
MgSO4 x 7 H2O 0.050 g 0.05 0.203
Na2S x 9 H2O 0.187 g 0.187 0.779
Vitamin solution 1.000 ml - -
Agar , for solid medium 15.000 g 15 44.599
Distilled water 1000.000 ml - -
1 Adjust to pH 7.3. Sterilize by autoclaving at 121°C for 15 minutes.
1154 GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)