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Main sol. 1160

Internal Solution-ID: S2326

Compound Amount Unit Conc. [g/L] Conc. [mM]
Casamino acids
(Difco)
7.50 g 7.5 -
Yeast extract 10.00 g 10 -
Trisodium citrate 3.00 g 3 11.625
KCl 2.00 g 2 26.828
MgSO4 x 7 H2O 20.00 g 20 81.144
FeCl2 x 4 H2O 36.00 mg 0.036 0.181
MnCl2 x 4 H2O 0.36 mg 3.6e-4 0.002
NaCl 250.00 g 250 4277.89
Distilled water 1000.00 ml - -
Agar , for solid medium 20.00 g 20 59.465
1 Adjust pH to 7.4. For solid medium add 20.0 g agar.
1160 PAYNE, SEGHAL & GIBBONS MEDIUM