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Main sol. 1161

Internal Solution-ID: S2327

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 0.5 g 0.5 5.883
K2HPO4 1.0 g 1 5.741
MgSO4 x 7 H2O 0.5 g 0.5 2.029
KCl 0.5 g 0.5 6.707
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
1 Adjust to pH 7.2. Autoclave at 121°C/15 min. Dispense into slopes. When the agar has solidified, place a strip of sterile filter paper on to the surface of each slope. Inoculate on to the filter paper.
1161 DUBOS SALTS MEDIUM