Main sol. 135b
Internal Solution-ID: S233
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.00 | g | 0.994 | 18.584 | |
KH2PO4 | 0.33 | g | 0.328 | 2.41 | |
K2HPO4 | 0.45 | g | 0.447 | 2.568 | |
MgSO4 x 7 H2O | 0.10 | g | 0.099 | 0.403 | |
Modified Wolin's mineral solution | 20.00 | ml | - | - | |
Yeast extract | 2.00 | g | 1.988 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Na2CO3 | 1.50 | g | 1.491 | 14.068 | |
Na-syringate solution (0.3 M) | 15.00 | ml | - | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.50 | g | 0.497 | 2.83 | |
Distilled water | 970.00 | ml | - | - | |
1 Dissolve ingredients except for carbonate, Na-syringate solution, vitamins, and cysteine, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Thereafter, dispense under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add Na-syringate solution, vitamins, and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the completed medium should be at 7.4. | |||||
2 Note: After growth has been started add an additional amount of Na-syringate to reach a final concentration of 2.00 g/l. |
135b | ACETOBACTERIUM DEHALOGENANS MEDIUM |