Main sol. 1166
Internal Solution-ID: S2337
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 6.00 | g | 5.982 | 102.362 | |
| K2HPO4 | 1.00 | g | 0.997 | 5.724 | |
| Na2SO4 | 2.80 | g | 2.792 | 19.654 | |
| NaHCO3 | 8.00 | g | 7.976 | 94.945 | |
| Na2CO3 | 22.00 | g | 21.934 | 206.95 | |
| NH4Cl | 0.20 | g | 0.199 | 3.728 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.199 | 0.981 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 0.05 | g | 0.05 | - | |
| Na-formate | 3.40 | g | 3.39 | 49.845 | |
| Na-pyruvate | 0.55 | g | 0.548 | 4.983 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 4.985 | 20.086 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.24 | g | 0.239 | 0.996 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve sodium chloride, hydrogenphosphate and sodium sulfate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, formate, pyruvate, thiosulfate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of vitamins and thiosulfate should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 10. | |||||
Media with this solution
| 1166 | DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM |