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Main sol. 136

Internal Solution-ID: S235

Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase
(BBL)
5.00 g 5 -
Yeast extract 3.00 g 3 -
Na-(DL)-lactate 7.50 g 7.5 -
Na-thioglycolate 0.75 g 0.75 6.573
Tween 80 1.00 ml - -
Glucose 1.00 g 1 5.551
Putrescine 3.00 mg 0.003 0.034
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5 with solid K2CO3.
136 VEILLONELLA MEDIUM