Main sol. 1200
Internal Solution-ID: S2389
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 10.00 | ml | - | - | |
Solution B | 1.00 | ml | - | - | |
Solution C | 0.20 | ml | - | - | |
Solution D ("red mix") | 0.20 | ml | - | - | |
Solution E (electron acceptor) | 0.05 | ml | - | - | |
Solution F: Vitamin solution according to Wolin and coauthors | 0.20 | ml | - | - | |
1 Prepare solutions A, B, and D separately and anaerobically under 80% N2 + 20% CO2 and autoclave. Filter-sterilize solutions C, E and F and outgas with N2. After cooling, add about 1 ml solution B per 10 ml solution A, by this way adjusting pH to 7.2. Add 0.2 ml solution C, 0.2 ml solution D, 0.2 ml solution F and 0.05 ml solution E, each per 10 ml solution A. |
1200 | ANAEROMYXOBACTER-MEDIUM |