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Main sol. 1202

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Internal Solution-ID: S2401

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.50 g 0.499 2.862
KH2PO4 0.50 g 0.499 3.663
NH4Cl 0.50 g 0.499 9.32
NaCl 0.40 g 0.399 6.824
KCl 0.10 g 0.1 1.337
Na2SO4 2.80 g 2.792 19.654
CaCl2 x 2 H2O 0.05 g 0.05 0.339
MgCl2 x 6 H2O 0.30 g 0.299 1.471
Yeast extract 0.10 g 0.1 -
Na-lactate 1.20 g 1.196 10.677
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.496 14.11
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.249 1.419
Na2S x 9 H2O 0.25 g 0.249 1.038
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Sterilize vitamins by filtration. Adjust pH of the complete medium to 7.0, if necessary.
1202 SOLIDESULFOVIBRIO MARRAKECHENSIS MEDIUM