Main sol. 1205
Internal Solution-ID: S2405
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BD BACTO) |
13.75 | g | 13.75 | - | |
| Yeast extract | 1.00 | g | 1 | - | |
| NaCl | 2.50 | g | 2.5 | 42.779 | |
| D-Glucose | 3.60 | g | 3.6 | 19.982 | |
|
DL-Dithiothreitol
(DTT) |
0.15 | g | 0.15 | 0.972 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except glucose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.2 with HCl, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Add glucose and DTT from anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration. | |||||
Media with this solution
| 1205 | 1/2 TRYPTIC SOY BROTH (ANAEROBIC) |