Main sol. 1206
Internal Solution-ID: S2406
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.27 | g | 0.267 | 1.962 | |
NH4Cl | 0.50 | g | 0.495 | 9.246 | |
(NH4)2SO4 | 0.50 | g | 0.495 | 3.743 | |
TAPS sodium salt
(SIGMA) |
2.40 | g | 2.374 | - | |
MgCl2 x 6 H2O | 0.01 | g | 0.01 | 0.049 | |
CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.135 | |
Yeast extract | 1.00 | g | 0.989 | - | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
NaHCO3 | 1.50 | g | 1.484 | 17.661 | |
D-Xylose | 3.00 | g | 2.967 | 19.765 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.25 | g | 0.247 | 1.408 | |
Na2S x 9 H2O | 0.25 | g | 0.247 | 1.03 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except bicarbonate, xylose, vitamins, cysteine and sulfide), adjust pH to 8.5, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add xylose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Sterilize stock solutions of xylose and vitamins by filtration. Adjust pH of the complete medium to 8.5, if necessary. |
1206 | CALDANAEROVIRGA MEDIUM |