Main sol. 141a
Internal Solution-ID: S243
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.34 | g | 0.336 | 4.502 | |
MgCl2 x 6 H2O | 4.00 | g | 3.949 | 19.423 | |
MgSO4 x 7 H2O | 3.45 | g | 3.406 | 13.818 | |
NH4Cl | 0.25 | g | 0.247 | 4.614 | |
CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.94 | |
K2HPO4 | 0.14 | g | 0.138 | 0.793 | |
NaCl | 18.00 | g | 17.769 | 304.055 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | - | |
Na-acetate | 1.00 | g | 0.987 | 12.034 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.50 | g | 0.494 | 2.81 | |
Na2S x 9 H2O | 0.50 | g | 0.494 | 2.055 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except vitamins, cysteine, and sulfide), sparge medium with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust the pH of the final medium to 6.2. | |||||
2 For incubation use sterile 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. |
141a | METHANOCALDOCOCCUS LAUENSIS MEDIUM |