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Main sol. 58

Internal Solution-ID: S2435

Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.00 g 10.05 -
2 5.00 g 5.025 -
3 5.00 g 5.025 -
4 5.00 g 5.025 -
5 10.00 g 10.05 55.786
6 2.00 g 2.01 11.54
7 0.20 g 0.201 0.816
8 0.05 g 0.05 0.297
9 1.00 ml - -
10 5.00 g 5.025 85.984
11 0.50 g 0.503 2.861
12 Salt solution 40.00 ml - -
13
(25 mg/100ml)
4.00 ml - -
14 950.00 ml 953.062 52902.9
15 , for pH adjustment x - -
Instructions:
  1. The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.
58 BIFIDOBACTERIUM MEDIUM