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Artificial seawater

Internal Solution-ID: S2453

Equipment needed: Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 27.7 473.99
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.60 g 0.6 8.048
NaBr 0.10 g 0.1 0.972
H3BO3 0.03 g 0.03 0.485
CaCl2 x 2 H2O 1.50 g 1.5 10.203
SrCl2 x 6 H2O 15.00 mg 0.015 0.056
KI 0.05 mg 5.0e-5 1.9e-4
Double distilled water 1000.00 ml - -
1 Adjust pH to 5.5 - 6.0.
1231 HALOPLASMA CONTRACTILE MEDIUM