Main sol. 1234
Internal Solution-ID: S2456
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 1.40 | g | 1.4 | 9.856 | |
| (NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| K2HPO4 | 0.05 | g | 0.05 | 0.287 | |
|
Ca(NO3)2 x 4 H2O
(0.1% w/v) |
20.00 | ml | 0.02 | 0.085 | |
|
(NH4)2Ni(SO4)2 x 6 H2O
(0.1% w/v) |
20.00 | ml | 0.02 | 0.051 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| K2S4O6 | 0.80 | g | 0.8 | - | |
| FeSO4 x 7 H2O | 0.03 | g | 0.03 | 0.108 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Dissolve ingredients (except tetrathionate and ferrous sulfate), adjust pH to 2.5 with H2SO4 and autoclave. After cooling, add tetrathionate from a 100 mM stock solution and FeSO4 from a 10 mM stock solution (prepared in water adjusted to pH 2.0 with H2SO4). Both solutions should be freshly prepared and sterilized by filtration. | |||||
Media with this solution
| 1234 | ACIDITHIOBACILLUS FERRIVORANS MEDIUM |